Difference between revisions of "Part:BBa K4361313"

 
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A mutant of the BlcR protein, created through site-directed mutagenesis with primers R3 ([[Part:BBa_K4361218]]) and F3.4 A67Q ([[Part:BBa_K4361222]]). For this mutant, the alanine in position 67 has been changed to glutamine by mutating the GCG codon to CAG.
 
A mutant of the BlcR protein, created through site-directed mutagenesis with primers R3 ([[Part:BBa_K4361218]]) and F3.4 A67Q ([[Part:BBa_K4361222]]). For this mutant, the alanine in position 67 has been changed to glutamine by mutating the GCG codon to CAG.
  
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This mutant also contains the following nucleotide mutations outside of the targeted site:
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This mutant contains no nucleotide substitutions or indels outside of the targeted site.
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Revision as of 14:09, 22 September 2022


BlcR A67Q

A mutant of the BlcR protein, created through site-directed mutagenesis with primers R3 (Part:BBa_K4361218) and F3.4 A67Q (Part:BBa_K4361222). For this mutant, the alanine in position 67 has been changed to glutamine by mutating the GCG codon to CAG.

This mutant contains no nucleotide substitutions or indels outside of the targeted site.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 694
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 78
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 589