Difference between revisions of "Part:BBa K4361309"

 
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A mutant of the BlcR protein, created through site-directed mutagenesis with primers R7 ([[Part:BBa_K4361210]]) and F7.7 H63Y ([[Part:BBa_K4361217]]). For this mutant, the histidine in position 63 has been changed to tyrosine by mutating the CAC codon to TAT.
 
A mutant of the BlcR protein, created through site-directed mutagenesis with primers R7 ([[Part:BBa_K4361210]]) and F7.7 H63Y ([[Part:BBa_K4361217]]). For this mutant, the histidine in position 63 has been changed to tyrosine by mutating the CAC codon to TAT.
  
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This mutant also contains the following nucleotide mutations outside of the targeted site:
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This mutant contains no nucleotide substitutions or indels outside of the targeted site.
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Revision as of 13:57, 22 September 2022


BlcR H63Y

A mutant of the BlcR protein, created through site-directed mutagenesis with primers R7 (Part:BBa_K4361210) and F7.7 H63Y (Part:BBa_K4361217). For this mutant, the histidine in position 63 has been changed to tyrosine by mutating the CAC codon to TAT.

This mutant contains no nucleotide substitutions or indels outside of the targeted site.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 694
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 78
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 589