Difference between revisions of "Part:BBa K4361303"
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Revision as of 13:04, 22 September 2022
BlcR S61V
A mutant of the BlcR protein, created through site-directed mutagenesis with primers R7 (Part:BBa_K4361210) and F7.1 S61V (Part:BBa_K4361211). For this mutant, the serine in position 61 has been changed to valine by mutating the AGT codon to GTG.
This mutant also contains the following nucleotide mutations outside of the targeted site:
- C 705 > T, silent mutation
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 694
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 78
- 1000INCOMPATIBLE WITH RFC[1000]Illegal SapI.rc site found at 589