Difference between revisions of "Part:BBa K4361300"

 
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  <li>G 944 > A, resulting in substitution E255K</li>
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  <li>G 763 > A, resulting in substitution E255K</li>
 
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Revision as of 12:04, 19 September 2022


BlcR D37R

A mutant of the BlcR protein, created through site-directed mutagenesis with primers R1 (BBa_K4361200) and F1.1 D37R (BBa_K4361201). For this mutant, the aspartic acid in position 37 has been changed to arginine.

This mutant also contains the following nucleotide mutations outside of the targeted site:

  • G 763 > A, resulting in substitution E255K

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 694
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 78
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 589