Difference between revisions of "Part:BBa K4151019"
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<partinfo>BBa_K4151019 short</partinfo> | <partinfo>BBa_K4151019 short</partinfo> | ||
| − | + | F2A is one of the members of the 2A self-cleaving peptide family, a class of 18–22 aa-long peptides, which can induce ribosomal skipping during the translation of a protein in a cell. F2A separates our target protein and RFP during transcription. If the sequence conjugates, it might form a fusion protein which can interrupt the process of protein folding and therefore affect the protein's function. By inserting F2A between RFP and AID, we ensure that fusion protein is less likely to be expressed, which leads to a higher possibility of properly folded AID. | |
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Latest revision as of 12:15, 11 October 2022
F2A
F2A is one of the members of the 2A self-cleaving peptide family, a class of 18–22 aa-long peptides, which can induce ribosomal skipping during the translation of a protein in a cell. F2A separates our target protein and RFP during transcription. If the sequence conjugates, it might form a fusion protein which can interrupt the process of protein folding and therefore affect the protein's function. By inserting F2A between RFP and AID, we ensure that fusion protein is less likely to be expressed, which leads to a higher possibility of properly folded AID.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]