Difference between revisions of "Part:BBa K4158008:Design"

(→‎Design Notes)
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===References===
 
===References===
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[1] Liu, X., Silverman, A. D., Alam, K. K., Iverson, E., Lucks, J. B., Jewett, M. C., and Raman, S. (2020) Design of a Transcriptional Biosensor for the Portable, On-Demand Detection of Cyanuric Acid. ACS Synth. Biol. 9 (1), 84– 94

Revision as of 08:07, 24 September 2022


RBS-AtzR


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 415
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 415
    Illegal NotI site found at 701
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 415
    Illegal BamHI site found at 151
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 415
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 415
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

If you want to get same plasmid that we experimented with, construct the plasmid along this way below.

1. Prepare pJL1 cloning vector (addgene Plasmid #69496)and this part as BioBrick.

2. Restriction and insertion cloning with pJL1 and this BioBrick productions and restriction enzymes XbaI and salI.

Source

test

References

[1] Liu, X., Silverman, A. D., Alam, K. K., Iverson, E., Lucks, J. B., Jewett, M. C., and Raman, S. (2020) Design of a Transcriptional Biosensor for the Portable, On-Demand Detection of Cyanuric Acid. ACS Synth. Biol. 9 (1), 84– 94