Difference between revisions of "Part:BBa K4180007"
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This pG1,10-snf1Δ381-633aa is a composite biobrick. pGal1, 10-SPT5-Streptavidin Binding Protein(SBP) plasmid, containing 2u origin of replication (ORI) for yeast to start DNA replication, and ORI for bacteria DNA replication, was sponsored by Dr. Tien-Hsien Chang, at Genomics Research Center, Academia Sinica, in Taipei Taiwan. This plasmid can be transformed into bacteria and yeast, which was beneficial for our team to finish making biobricks in bacteria and perform the functional assay in yeast. Our team made 5 different basic parts (one promoter and 4 coding regions) and 4 different composite parts by inserting those 4 basic parts (BBa_K4180000, BBa_K4180002, BBa_K4180003, and BBa_K4180004) to replace the original SPT5 gene. pGal1,10-snf1Δ381-633, C-terminus truncated from amino acid381 to 633 deleting the autoinhibitory domain and SIP-interacting domain (SIR), losing the interaction with downstream of proteins. | This pG1,10-snf1Δ381-633aa is a composite biobrick. pGal1, 10-SPT5-Streptavidin Binding Protein(SBP) plasmid, containing 2u origin of replication (ORI) for yeast to start DNA replication, and ORI for bacteria DNA replication, was sponsored by Dr. Tien-Hsien Chang, at Genomics Research Center, Academia Sinica, in Taipei Taiwan. This plasmid can be transformed into bacteria and yeast, which was beneficial for our team to finish making biobricks in bacteria and perform the functional assay in yeast. Our team made 5 different basic parts (one promoter and 4 coding regions) and 4 different composite parts by inserting those 4 basic parts (BBa_K4180000, BBa_K4180002, BBa_K4180003, and BBa_K4180004) to replace the original SPT5 gene. pGal1,10-snf1Δ381-633, C-terminus truncated from amino acid381 to 633 deleting the autoinhibitory domain and SIP-interacting domain (SIR), losing the interaction with downstream of proteins. | ||
| − | [[File:BBa_K4180007.jpeg |200px|thumb| | + | [[File:BBa_K4180007.jpeg |200px|thumb|right|]] |
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Revision as of 06:01, 15 September 2022
pG1,10-snf1Δ381-633aa
This pG1,10-snf1Δ381-633aa is a composite biobrick. pGal1, 10-SPT5-Streptavidin Binding Protein(SBP) plasmid, containing 2u origin of replication (ORI) for yeast to start DNA replication, and ORI for bacteria DNA replication, was sponsored by Dr. Tien-Hsien Chang, at Genomics Research Center, Academia Sinica, in Taipei Taiwan. This plasmid can be transformed into bacteria and yeast, which was beneficial for our team to finish making biobricks in bacteria and perform the functional assay in yeast. Our team made 5 different basic parts (one promoter and 4 coding regions) and 4 different composite parts by inserting those 4 basic parts (BBa_K4180000, BBa_K4180002, BBa_K4180003, and BBa_K4180004) to replace the original SPT5 gene. pGal1,10-snf1Δ381-633, C-terminus truncated from amino acid381 to 633 deleting the autoinhibitory domain and SIP-interacting domain (SIR), losing the interaction with downstream of proteins.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 1192
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 286
- 1000COMPATIBLE WITH RFC[1000]