Difference between revisions of "Part:BBa K190018:Design"
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gi|81857196|sp|Q9I147|Q9I147_PSEAE | gi|81857196|sp|Q9I147|Q9I147_PSEAE | ||
| − | Derived from construct used for paper: | + | Derived from construct used for paper by: |
| − | Lewinson | + | Dr. Oded Lewinson |
| + | Howard Hughes Medical Institute and Division of Chemistry and Chemical Engineering, MC 114-96 | ||
| + | California Institute of Technology | ||
===References=== | ===References=== | ||
Lewinson O., Lee A.T., Rees D.C. 2009. A P-type ATPase importer that discriminates between essential and toxic transition metals. PNAS. vol. 106, no. 12, p. 4677-468 | Lewinson O., Lee A.T., Rees D.C. 2009. A P-type ATPase importer that discriminates between essential and toxic transition metals. PNAS. vol. 106, no. 12, p. 4677-468 | ||
Latest revision as of 10:06, 3 August 2009
HmtA
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 1138
- 21INCOMPATIBLE WITH RFC[21]Illegal XhoI site found at 646
Illegal XhoI site found at 1009 - 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 559
Illegal NgoMIV site found at 1286
Illegal NgoMIV site found at 1460
Illegal NgoMIV site found at 1675
Illegal NgoMIV site found at 1736
Illegal NgoMIV site found at 1763
Illegal NgoMIV site found at 1772 - 1000COMPATIBLE WITH RFC[1000]
Design Notes
Still contains a EcoRI site at position 6.
Two PstI sites have been removed
Source
gi|81857196|sp|Q9I147|Q9I147_PSEAE
Derived from construct used for paper by:
Dr. Oded Lewinson Howard Hughes Medical Institute and Division of Chemistry and Chemical Engineering, MC 114-96 California Institute of Technology
References
Lewinson O., Lee A.T., Rees D.C. 2009. A P-type ATPase importer that discriminates between essential and toxic transition metals. PNAS. vol. 106, no. 12, p. 4677-468