Difference between revisions of "Part:BBa K4377000:Design"

 
(→‎Design Notes)
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According to Escherichia coli codon preference.
 
According to Escherichia coli codon preference.
  
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+
In order to purify our protein after expression, we added 10x His tag at the N terminal of Ubx protein. The reason we add 10x His tag at N terminal but not C terminal is to amplify the production of Ubx protein. To improve performance, we performed codon harmonization. The optimized sequence of codons will be more suitable for expression by our engineered E. coli
  
 
===Source===
 
===Source===

Revision as of 19:39, 11 October 2022


Ubx


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NotI site found at 103
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 316
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

According to Escherichia coli codon preference.

In order to purify our protein after expression, we added 10x His tag at the N terminal of Ubx protein. The reason we add 10x His tag at N terminal but not C terminal is to amplify the production of Ubx protein. To improve performance, we performed codon harmonization. The optimized sequence of codons will be more suitable for expression by our engineered E. coli

Source

Drosophila melanogaster, sequence found through Uniprot https://www.uniprot.org/uniprotkb/P83949/entry

References