Difference between revisions of "Part:BBa K4235003:Design"

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===Design Notes===
 
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Revision as of 23:12, 12 September 2022


Gentamicin Resistance Gene


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 314
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Source

This gentamicin coding region is from the mini-attTn7 segment, part of the pFastBac-Htb_miniTn7 vector

References

Doi, Y., Wachino, J. I., & Arakawa, Y. (2016). Aminoglycoside Resistance: The Emergence of Acquired 16S Ribosomal RNA Methyltransferases. Infectious disease clinics of North America, 30(2), 523–537. https://doi.org/10.1016/j.idc.2016.02.011