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Revision as of 23:05, 12 September 2022

Gentamicin Resistance Gene

Usage and Biology

Introduction: Gentamicin is an aminoglycoside frequently used in clinical settings to treat many bacterial infections. A gentamicin resistance gene can confer resistance to aminoglycosides based on several mechanisms: enzymatic inactivation of aminoglycosides, increased efflux from the bacterial cell, interfering with binding domains of aminoglycosides, etc.

Usage and Biology:

This part is the entire circuit of the Gentamicin Resistance gene which is used within the plasmid (pFastBac-YmBac2) and it confers resistance to gentamicin. The expression of the gentamicin coding sequence is driven by the pC promoter (add link). This circuit is also a part of the mini-attTn7 transposon segment (add link), used in the Bac-to-Bac expression system by Stony_Brook 2021.

This marker is used to provide gentamicin resistance to the pFastBac vector during the transposition in the Bacmid, propagated in E coli DH10Bac cells. A concentration of 7 ug/ml gentamicin is recommended for transposition.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 314
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Source

This Gentamicin resistance gene is part of the original plasmid pFastBac, which was a kind gift from the Airola lab at Stony Brook University.