Difference between revisions of "Part:BBa K4361200"
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<partinfo>BBa_K4361200 short</partinfo> | <partinfo>BBa_K4361200 short</partinfo> | ||
− | For the site-directed mutagenesis (SDM) of our protein of interest, BlcR, multiple sets of back-to-back primers were designed. The 5' ends of these primers anneal to adjacent nucleotides such that the full plasmid containing the protein (<a href="https://parts.igem.org/ | + | For the site-directed mutagenesis (SDM) of our protein of interest, BlcR, multiple sets of back-to-back primers were designed. The 5' ends of these primers anneal to adjacent nucleotides such that the full plasmid containing the protein (<a href="https://parts.igem.org/">BBa_K4361106</a>) can be replicated. Each of these sets of primers contains a single reverse primer, denoted as "primer R<i>n</i>" with <i>n</i> being the number of the set, and a number of forward primers, in this case F1.1 ([https://parts.igem.org/Part:BBa_K4361201 BBa_K4361201]) through F1.3 ([https://parts.igem.org/Part:BBa_K4361203 BBa_K4361203]). This set covers amino acids 36 to 39, although 36 is not represented in our BioBricks due to that SDM experiment being unsuccesful. |
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Revision as of 07:42, 12 September 2022
BlcR BTB primer R1
For the site-directed mutagenesis (SDM) of our protein of interest, BlcR, multiple sets of back-to-back primers were designed. The 5' ends of these primers anneal to adjacent nucleotides such that the full plasmid containing the protein (<a href="https://parts.igem.org/">BBa_K4361106</a>) can be replicated. Each of these sets of primers contains a single reverse primer, denoted as "primer Rn" with n being the number of the set, and a number of forward primers, in this case F1.1 (BBa_K4361201) through F1.3 (BBa_K4361203). This set covers amino acids 36 to 39, although 36 is not represented in our BioBricks due to that SDM experiment being unsuccesful.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]