Difference between revisions of "Part:BBa K4361200"

Revision as of 07:40, 12 September 2022

BlcR BTB primer R1

For the site-directed mutagenesis (SDM) of our protein of interest, BlcR, multiple sets of back-to-back primers were designed. The 5' ends of these primers anneal to adjacent nucleotides such that the full plasmid containing the protein (<a href="https://parts.igem.org/Part:BBa_K4361106;">BBa_K4361106</a>) can be replicated. Each of these sets of primers contains a single reverse primer, denoted as "primer Rn" with n being the number of the set, and a number of forward primers, in this case F1.1 (BBa_K4361201) through F1.3 (BBa_K4361203). This set covers amino acids 36 to 39, although 36 is not represented in our BioBricks due to that SDM experiment being unsuccesful.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

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 <partinfo>BBa_K4361200 short</partinfo>
-For the site-directed mutagenesis (SDM) of our protein of interest, BlcR, multiple sets of back-to-back primers were designed. The 5' ends of these primers anneal to adjacent nucleotides such that the full plasmid containing the protein (<a href="https://parts.igem.org/Part:BBa_K4361106">BBa_K4361106</a>) can be replicated. Each of these sets of primers contains a single reverse primer, denoted as "primer R<i>n</i>" with <i>n</i> being the number of the set, and a number of forward primers, in this case F1.1 ([https://parts.igem.org/Part:BBa_K4361201 BBa_K4361201]) through F1.3 ([https://parts.igem.org/Part:BBa_K4361203 BBa_K4361203]). This set covers amino acids 36 to 39, although 36 is not represented in our BioBricks due to that SDM experiment being unsuccesful.
+For the site-directed mutagenesis (SDM) of our protein of interest, BlcR, multiple sets of back-to-back primers were designed. The 5' ends of these primers anneal to adjacent nucleotides such that the full plasmid containing the protein (<a href="https://parts.igem.org/Part:BBa_K4361106;">BBa_K4361106</a>) can be replicated. Each of these sets of primers contains a single reverse primer, denoted as "primer R<i>n</i>" with <i>n</i> being the number of the set, and a number of forward primers, in this case F1.1 ([https://parts.igem.org/Part:BBa_K4361201 BBa_K4361201]) through F1.3 ([https://parts.igem.org/Part:BBa_K4361203 BBa_K4361203]). This set covers amino acids 36 to 39, although 36 is not represented in our BioBricks due to that SDM experiment being unsuccesful.
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