Difference between revisions of "Part:BBa K4137000"
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<partinfo>BBa_K4137000 short</partinfo>
 
<partinfo>BBa_K4137000 short</partinfo>
  
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BBa_K4137000, nicknamed ccdB for the purpose of our project, encodes for the production of CcdB protein which is a toxin to many bacteria. CcdB loses its toxicity by binding with CcdA, which prevents it from binding and interact with DNA Gyrase. The Myc tag was added to the CcdB coding sequence during the part design, this allows us to characterize the protein and go through purification.  
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BBa_K4137000 codes for the CcdA protein, a component of the CcdA-CcdB toxin-antitoxin system. CcdA binds with CcdB which inhibits CcdB from targeting DNA gyrase thus preventing DNA double-strand breaks. The 6xHis tag is used for protein purification and identification.  
  
 
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Revision as of 06:16, 5 October 2022


CcdA-6xHis Antitoxin

BBa_K4137000 codes for the CcdA protein, a component of the CcdA-CcdB toxin-antitoxin system. CcdA binds with CcdB which inhibits CcdB from targeting DNA gyrase thus preventing DNA double-strand breaks. The 6xHis tag is used for protein purification and identification.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]