Difference between revisions of "Part:BBa K4477002"

 
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<partinfo>BBa_K4477002 short</partinfo>
 
<partinfo>BBa_K4477002 short</partinfo>
  
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This sequence codes for the heavy chain of an anti-apoB(MDA) immunoglobulin G (IgG). ApoB(MDA) is a specific chemical structure found as a component of oxidized low density lipoprotein (oxLDL). OxLDL is a biomarker of many inflammatory diseases such as atherosclerosis, so antibodies able to target oxLDL could enable potential detection and therapeutic applications for such diseases. This part is best used when expressed as part of the same expression cassette with part BBa_K4477001, which encodes the light chain of this anti-oxLDL antibody. When expressed together, the light and heavy chains will associate to form a complete IgG. See part [NAME] for the composite part Team Virginia 2022 recommends for expressing both light and heavy chains together.
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This sequence codes for the heavy chain of an anti-apoB(MDA) immunoglobulin G (IgG). ApoB(MDA) is a specific chemical structure found as a component of oxidized low density lipoprotein (oxLDL). OxLDL is a biomarker of many inflammatory diseases such as atherosclerosis, so antibodies able to target oxLDL could enable potential detection and therapeutic applications for such diseases. This part is best used when expressed as part of the same expression cassette with part BBa_K4477001, which encodes the light chain of this anti-oxLDL antibody. When expressed together, the light and heavy chains will associate to form a complete IgG. See part BBa_K4477011 for the composite part Team Virginia 2022 recommends for expressing both light and heavy chains together.
  
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Like any antibody, Team Virginia 2022's Orticumab biosimilar requires disulfide bonds for it to be properly folded and exhibit the correct binding affinity. As a result, it is crucial that both the light and heavy chain CDSs be expressed in an organism capable of creating disulfide bonds while it expresses proteins, such as the SHuffle bacterial strain or most eukaryotic organisms. Note that many common bacterial strains do not have this capability! Team Virginia 2022 used SHuffle bacteria to express this Orticumab biosimilar.
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Like any antibody, Team Virginia 2022's hybrid anti-apoB(MDA) IgG requires disulfide bonds for it to be properly folded and exhibit the correct binding affinity. As a result, it is crucial that both the light and heavy chain CDSs be expressed in an organism capable of creating disulfide bonds while it expresses proteins, such as the SHuffle bacterial strain or most eukaryotic organisms. Note that many common bacterial strains do not have this capability! Team Virginia 2022 used SHuffle <em> E. coli </em> to express this hybrid anti-apoB(MDA) IgG.
  
 
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===Functional Parameters===
 
===Functional Parameters===
 
<partinfo>BBa_K4477002 parameters</partinfo>
 
<partinfo>BBa_K4477002 parameters</partinfo>
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Revision as of 07:11, 12 October 2022


Human/mouse anti-apoB(MDA) (anti-oxLDL) IgG antibody - heavy chain

This sequence codes for the heavy chain of an anti-apoB(MDA) immunoglobulin G (IgG). ApoB(MDA) is a specific chemical structure found as a component of oxidized low density lipoprotein (oxLDL). OxLDL is a biomarker of many inflammatory diseases such as atherosclerosis, so antibodies able to target oxLDL could enable potential detection and therapeutic applications for such diseases. This part is best used when expressed as part of the same expression cassette with part BBa_K4477001, which encodes the light chain of this anti-oxLDL antibody. When expressed together, the light and heavy chains will associate to form a complete IgG. See part BBa_K4477011 for the composite part Team Virginia 2022 recommends for expressing both light and heavy chains together.

Like any antibody, Team Virginia 2022's hybrid anti-apoB(MDA) IgG requires disulfide bonds for it to be properly folded and exhibit the correct binding affinity. As a result, it is crucial that both the light and heavy chain CDSs be expressed in an organism capable of creating disulfide bonds while it expresses proteins, such as the SHuffle bacterial strain or most eukaryotic organisms. Note that many common bacterial strains do not have this capability! Team Virginia 2022 used SHuffle E. coli to express this hybrid anti-apoB(MDA) IgG.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 73
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 1158
  • 1000
    COMPATIBLE WITH RFC[1000]


Uncomment this to enable Functional Parameter display

Functional Parameters