Difference between revisions of "Part:BBa K4361103"

 
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<partinfo>BBa_K4361103 short</partinfo>
 
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TBD
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Composite part consisting of <i>E. coli</i> codon optimized BlcR ([[Part:BBa_K4361100]], with a His-tag for purification and TEV cleavage site for separation of said tag from the protein ([[Part:BBa_K4361104]]). Through this combination, we were able to attain improved expression of our protein of interest and subsequently purify it without inhibiting its functionality.
  
 
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<span class='h3bb'>Sequence and Features</span>
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<span class='h3bb'><h3>Sequence and Features</h3></span>
 
<partinfo>BBa_K4361103 SequenceAndFeatures</partinfo>
 
<partinfo>BBa_K4361103 SequenceAndFeatures</partinfo>
  

Revision as of 08:17, 11 October 2022


BlcR with 6xHis-tag and TEV protease cleavage site

Composite part consisting of E. coli codon optimized BlcR (Part:BBa_K4361100, with a His-tag for purification and TEV cleavage site for separation of said tag from the protein (Part:BBa_K4361104). Through this combination, we were able to attain improved expression of our protein of interest and subsequently purify it without inhibiting its functionality.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 766
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 901
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 150
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 661