Difference between revisions of "Part:BBa K4175001"

Revision as of 12:33, 27 August 2022

Notch core

This protein domain is derived from the transmembrane part of the Notch protein. When the extracellular domain of Notch binds to its ligand Delta, the Notch core domain will undergo intramembrane proteolysis, releasing the intracellular domain. The released intracellular domain acts as a transcriptional regulator, affecting the cellular activity. The extracellular and intracellular domain can be artificially swapped, offering a tool for designing novel signaling pathways [1].

Sequence and Features

Assembly Compatibility:

10
INCOMPATIBLE WITH RFC[10]
Illegal PstI site found at 115
Illegal PstI site found at 156
Illegal PstI site found at 202
12
INCOMPATIBLE WITH RFC[12]
Illegal PstI site found at 115
Illegal PstI site found at 156
Illegal PstI site found at 202
21
COMPATIBLE WITH RFC[21]
23
INCOMPATIBLE WITH RFC[23]
Illegal PstI site found at 115
Illegal PstI site found at 156
Illegal PstI site found at 202
25
INCOMPATIBLE WITH RFC[25]
Illegal PstI site found at 115
Illegal PstI site found at 156
Illegal PstI site found at 202
Illegal NgoMIV site found at 28
1000
COMPATIBLE WITH RFC[1000]

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 <!-- Add more about the biology of this part here
 ===Usage and Biology===
 ===References===
 [1] Morsut, L., Roybal, K.T., Xiong, X., Gordley, R.M., Coyle, S.M., Thomson, M., Lim, W.A., 2016. Engineering Customized Cell Sensing and Response Behaviors Using Synthetic Notch Receptors. Cell 164, 780–791. https://doi.org/10.1016/j.cell.2016.01.012
 <!-- -->
 <span class='h3bb'>Sequence and Features</span>