Difference between revisions of "Part:BBa K4175000:Design"

 
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===References===
 
===References===
 +
ADRIAN, T.H.J., DARIO, C., 2021. Neutralization of Human Cytokines with Membrane-Bound Anti-Cytokine Non-Signaling Binders Expressed in Immune Cells.

Revision as of 11:46, 27 August 2022


anti-IL-6 scFv


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 604
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 604
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 512
    Illegal XhoI site found at 496
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 604
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 604
    Illegal AgeI site found at 556
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The sequence has been condon optimized for human.


Source

Human. Sequence found in ADRIAN, T.H.J., DARIO, C., 2021. Neutralization of Human Cytokines with Membrane-Bound Anti-Cytokine Non-Signaling Binders Expressed in Immune Cells. https://www.lens.org/lens/patent/040-383-008-445-12X/citations?view=in

References

ADRIAN, T.H.J., DARIO, C., 2021. Neutralization of Human Cytokines with Membrane-Bound Anti-Cytokine Non-Signaling Binders Expressed in Immune Cells.