Difference between revisions of "Part:BBa K4370010:Experience"
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===Applications of BBa_K4370010=== | ===Applications of BBa_K4370010=== | ||
| − | BBa_K4370010 was | + | <partinfo>BBa_K4370010</partinfo> was introduced between NotI sites into the pOSV805 plasmid, replacing the initial cloning module that contains the blue chromoprotein encoding gene <i>amylCP</i> (<partinfo>BBa_K1155003</partinfo>) under the control of <i>E. coli </i> promoter (<partinfo>BBa_K4370009</partinfo>, not functional in <i>Streptomyces</i>). |
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| + | Since <partinfo>BBa_K4370010</partinfo> contains two promoters (one active in <i>Streptomyces</i>, one active in <i>E. coli</i>), we had to check first that there was no promoter interference and that the <i>mRFP1</i> gene could be properly expressed from this module at a level sufficient to allow a visual screening for further applications. | ||
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| + | Therefore, after cloning <partinfo>BBa_K4370010</partinfo> into pOSV805, we observed the color of the colonies on plate. We could clearly distinguish red and white colonies, harboring or not the <partinfo>BBa_K4370010</partinfo> part respectively. Thus, whereas bacteria pOV805 were blue, the bacteria transformed by pOSV805- BBa_K4370010 are now red. | ||
===User Reviews=== | ===User Reviews=== | ||
Revision as of 11:56, 22 August 2022
This experience page is provided so that any user may enter their experience using this part.
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how you used this part and how it worked out.
Applications of BBa_K4370010
BBa_K4370010 was introduced between NotI sites into the pOSV805 plasmid, replacing the initial cloning module that contains the blue chromoprotein encoding gene amylCP (BBa_K1155003) under the control of E. coli promoter (BBa_K4370009, not functional in Streptomyces).
Since BBa_K4370010 contains two promoters (one active in Streptomyces, one active in E. coli), we had to check first that there was no promoter interference and that the mRFP1 gene could be properly expressed from this module at a level sufficient to allow a visual screening for further applications.
Therefore, after cloning BBa_K4370010 into pOSV805, we observed the color of the colonies on plate. We could clearly distinguish red and white colonies, harboring or not the BBa_K4370010 part respectively. Thus, whereas bacteria pOV805 were blue, the bacteria transformed by pOSV805- BBa_K4370010 are now red.
User Reviews
UNIQce9ebf14d5789e89-partinfo-00000006-QINU UNIQce9ebf14d5789e89-partinfo-00000007-QINU