Difference between revisions of "Part:BBa K4137003"
Line 3: | Line 3: | ||
<partinfo>BBa_K4137003 short</partinfo> | <partinfo>BBa_K4137003 short</partinfo> | ||
− | This part consists of a secretion tag yebF (BBa_K2922002), a GS Linker (BBa_J18921), a chi18h8 coding sequence, and a 6xHistag to purify the produced protein. | + | This part consists of a secretion tag yebF (BBa_K2922002), a GS Linker (BBa_J18921), a chi18h8 coding sequence, and a 6xHistag to purify the produced protein. It is a chitinase expression and secretion construct. |
Revision as of 01:44, 8 October 2022
Chi18h8-YebF Fusion Protein
This part consists of a secretion tag yebF (BBa_K2922002), a GS Linker (BBa_J18921), a chi18h8 coding sequence, and a 6xHistag to purify the produced protein. It is a chitinase expression and secretion construct.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 1492
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 1005
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 552
- 1000COMPATIBLE WITH RFC[1000]