Difference between revisions of "Part:BBa K4370011"
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This part encodes a single guide RNA targeting one of the <i> lsr2 </i> paralogs (<i> lsr2A </i>) encoding the main xenogeneic silencer of <i> Streptomyces </i>. It has been designed to target different strains such as <i> S. ambofaciens </i> ATCC 23877, <i> S. bottropensis </i> ATCC 25435, <i> S. venezuelae </i> ATCC 10712 and <i> S. rimosus </i> ATCC 10970.
 
This part encodes a single guide RNA targeting one of the <i> lsr2 </i> paralogs (<i> lsr2A </i>) encoding the main xenogeneic silencer of <i> Streptomyces </i>. It has been designed to target different strains such as <i> S. ambofaciens </i> ATCC 23877, <i> S. bottropensis </i> ATCC 25435, <i> S. venezuelae </i> ATCC 10712 and <i> S. rimosus </i> ATCC 10970.
  
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Xenogeneic silencers of Lsr2 family have been shown to silence the expression of specialized metabolite biosynthetic gene clusters (Gerke et al., eLife, 2019 - doi:10.7554/eLife.47691 ; Zhang et al., Genetics and Molecular Biology, 2021 - https://journals.asm.org/doi/10.1128/mBio.01077-21 ; Deng et al., RSC Adv, 2017 - DOI: 10.1039/c7ra06482a).  
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===Usage and Biology===
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Xenogeneic silencers of Lsr2 family have been shown to silence the expression of specialized metabolite biosynthetic gene clusters (Gerke et al., eLife, 2019 ; Zhang et al., Genetics and Molecular Biology, 2021 ; Deng et al., RSC Adv, 2017).  
 
Thus decreasing their expression has been associated with increased production of specialized metabolites including antibiotics.
 
Thus decreasing their expression has been associated with increased production of specialized metabolites including antibiotics.
  
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<!-- Add more about the biology of this part here
 
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===Usage and Biology===
 
  
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<span class='h3bb'>Sequence and Features</span>
 
<span class='h3bb'>Sequence and Features</span>
 
<partinfo>BBa_K4370011 SequenceAndFeatures</partinfo>
 
<partinfo>BBa_K4370011 SequenceAndFeatures</partinfo>
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===References===
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Emma J Gehrke Xiafei Zhang Sheila M Pimentel-Elardo Andrew R Johnson Christiaan A Rees Stephanie E Jones Hindra Sebastian S Gehrke Sonya Turvey Suzanne Boursalie Jane E Hill Erin E Carlson Justin R Nodwell Marie A Elliot, “Silencing cryptic specialized metabolism in <i>Streptomyces</i> by the nucleoid-associated protein Lsr2” (2019) eLife 8:e47691.
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https://doi.org/10.7554/eLife.47691
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Xiafei Zhang, Sara N. Andres, Marie A. Elliot, “Interplay between Nucleoid-Associated Proteins and Transcription Factors in Controlling Specialized Metabolism in <i>Streptomyces</i>” (2021) mBio Vol. 12, No. 4
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https://doi.org/10.1128/mBio.01077-21
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Lina Deng,a  Rui Wang,a  Guowei Wang,*a  Mingxu Liu,a  Guojian Liao,a  Zhihua Liaob  and  Min Chen, “Targeted isolation of sulfur-containing metabolites from Lsr2-deletion mutant strain of <i>Streptomyces roseosporus</i>” (2017) RSC Adv., 2017,7, 37771-37777
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https://doi.org/10.1039/C7RA06482A
  
  

Revision as of 08:04, 22 August 2022


sgRNA_lsr2A_STREPTO

This part encodes a single guide RNA targeting one of the lsr2 paralogs ( lsr2A ) encoding the main xenogeneic silencer of Streptomyces . It has been designed to target different strains such as S. ambofaciens ATCC 23877, S. bottropensis ATCC 25435, S. venezuelae ATCC 10712 and S. rimosus ATCC 10970.

Usage and Biology

Xenogeneic silencers of Lsr2 family have been shown to silence the expression of specialized metabolite biosynthetic gene clusters (Gerke et al., eLife, 2019 ; Zhang et al., Genetics and Molecular Biology, 2021 ; Deng et al., RSC Adv, 2017). Thus decreasing their expression has been associated with increased production of specialized metabolites including antibiotics.


Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Unknown
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]

References

Emma J Gehrke Xiafei Zhang Sheila M Pimentel-Elardo Andrew R Johnson Christiaan A Rees Stephanie E Jones Hindra Sebastian S Gehrke Sonya Turvey Suzanne Boursalie Jane E Hill Erin E Carlson Justin R Nodwell Marie A Elliot, “Silencing cryptic specialized metabolism in Streptomyces by the nucleoid-associated protein Lsr2” (2019) eLife 8:e47691. https://doi.org/10.7554/eLife.47691 Xiafei Zhang, Sara N. Andres, Marie A. Elliot, “Interplay between Nucleoid-Associated Proteins and Transcription Factors in Controlling Specialized Metabolism in Streptomyces” (2021) mBio Vol. 12, No. 4 https://doi.org/10.1128/mBio.01077-21 Lina Deng,a Rui Wang,a Guowei Wang,*a Mingxu Liu,a Guojian Liao,a Zhihua Liaob and Min Chen, “Targeted isolation of sulfur-containing metabolites from Lsr2-deletion mutant strain of Streptomyces roseosporus” (2017) RSC Adv., 2017,7, 37771-37777 https://doi.org/10.1039/C7RA06482A