Difference between revisions of "Part:BBa K4370011"
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<partinfo>BBa_K4370011 short</partinfo> | <partinfo>BBa_K4370011 short</partinfo> | ||
| − | This part encodes a single guide RNA targeting one of the <i> lsr2 </i> paralogs (lsr2A) encoding the main xenogeneic silencer of <i> Streptomyces </i>. It has been designed to target different strains such as <i> S. ambofaciens </i> ATCC 23877, <i> S. bottropensis </i> ATCC 25435, <i> S. venezuelae </i> ATCC 10712 and <i> S. rimosus </i> ATCC 10970. | + | This part encodes a single guide RNA targeting one of the <i> lsr2 </i> paralogs (<i> lsr2A </i>) encoding the main xenogeneic silencer of <i> Streptomyces </i>. It has been designed to target different strains such as <i> S. ambofaciens </i> ATCC 23877, <i> S. bottropensis </i> ATCC 25435, <i> S. venezuelae </i> ATCC 10712 and <i> S. rimosus </i> ATCC 10970. |
Xenogeneic silencers of Lsr2 family have been shown to silence the expression of specialized metabolite biosynthetic gene clusters (Gerke et al., eLife, 2019 - doi:10.7554/eLife.47691 ; Zhang et al., Genetics and Molecular Biology, 2021 - https://journals.asm.org/doi/10.1128/mBio.01077-21 ; Deng et al., RSC Adv, 2017 - DOI: 10.1039/c7ra06482a). | Xenogeneic silencers of Lsr2 family have been shown to silence the expression of specialized metabolite biosynthetic gene clusters (Gerke et al., eLife, 2019 - doi:10.7554/eLife.47691 ; Zhang et al., Genetics and Molecular Biology, 2021 - https://journals.asm.org/doi/10.1128/mBio.01077-21 ; Deng et al., RSC Adv, 2017 - DOI: 10.1039/c7ra06482a). | ||
Revision as of 14:23, 18 August 2022
sgRNA_lsr2A_STREPTO
This part encodes a single guide RNA targeting one of the lsr2 paralogs ( lsr2A ) encoding the main xenogeneic silencer of Streptomyces . It has been designed to target different strains such as S. ambofaciens ATCC 23877, S. bottropensis ATCC 25435, S. venezuelae ATCC 10712 and S. rimosus ATCC 10970.
Xenogeneic silencers of Lsr2 family have been shown to silence the expression of specialized metabolite biosynthetic gene clusters (Gerke et al., eLife, 2019 - doi:10.7554/eLife.47691 ; Zhang et al., Genetics and Molecular Biology, 2021 - https://journals.asm.org/doi/10.1128/mBio.01077-21 ; Deng et al., RSC Adv, 2017 - DOI: 10.1039/c7ra06482a). Thus decreasing their expression has been associated with increased production of specialized metabolites including antibiotics.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]