Difference between revisions of "Part:BBa K4370010:Design"
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===Design Notes=== | ===Design Notes=== | ||
| − | The NdeI site (catATG) allows the easy cloning of genes between NdeI (ATG/start codon) and SpeI sites (this latter site been in the suffixe). | + | The NdeI site (catATG) allows the easy cloning of genes between NdeI (ATG/start codon) and SpeI sites (this latter site been in the suffixe). This module is also designed to be easily cloned between NotI sites in the collection of <i> Streptomyces </i> integrative vectors published by Aubry and collaborators (Appl Environ Microbiol, 2019). |
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Wang et al., Appl Environ Microbiol, 2013 - https://journals.asm.org/doi/10.1128/AEM.00985-13 | Wang et al., Appl Environ Microbiol, 2013 - https://journals.asm.org/doi/10.1128/AEM.00985-13 | ||
| − | Aubry et al., Appl Environ Microbiol, | + | Aubry et al., Appl Environ Microbiol, 2019 - https://journals.asm.org/doi/10.1128/AEM.00485-19 |
Revision as of 13:39, 18 August 2022
STREPTO_E.coli_CLONING_MODULE
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23INCOMPATIBLE WITH RFC[23]Unknown
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 792
Illegal AgeI site found at 904 - 1000COMPATIBLE WITH RFC[1000]
Design Notes
The NdeI site (catATG) allows the easy cloning of genes between NdeI (ATG/start codon) and SpeI sites (this latter site been in the suffixe). This module is also designed to be easily cloned between NotI sites in the collection of Streptomyces integrative vectors published by Aubry and collaborators (Appl Environ Microbiol, 2019).
Source
The sequence has been synthetized.
References
Bai et al., PNAS, 2015 - www.pnas.org/cgi/doi/10.1073/pnas.1511027112
Wang et al., Appl Environ Microbiol, 2013 - https://journals.asm.org/doi/10.1128/AEM.00985-13
Aubry et al., Appl Environ Microbiol, 2019 - https://journals.asm.org/doi/10.1128/AEM.00485-19