Difference between revisions of "Part:BBa K4370000:Design"

 
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===Design Notes===
 
===Design Notes===
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The sequence of the native gene STRBO_RS0123595 of Streptomyces bottropensis ATCC 25435 has been codon optimized for an expression in E. coli. Indeed, the Streptomyces genomes are GC rich (circa 72%), which requires extensive sequence optimisation for production in E. coli.
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The sequence of the native gene STRBO_RS0123595 of Streptomyces bottropensis ATCC 25435 has been codon optimized for an expression in E. coli. Indeed, the Streptomyces genomes are GC rich (circa 72%), which requires extensive sequence optimization for expression in E. coli.
  
  

Revision as of 14:30, 17 August 2022


cbbL_STRBO


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 373


Design Notes

The sequence of the native gene STRBO_RS0123595 of Streptomyces bottropensis ATCC 25435 has been codon optimized for an expression in E. coli. Indeed, the Streptomyces genomes are GC rich (circa 72%), which requires extensive sequence optimization for expression in E. coli.


Source

This sequence has be synthetized.

References