Difference between revisions of "Part:BBa K4390034"

 
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<partinfo>BBa_K4390034 short</partinfo>
 
<partinfo>BBa_K4390034 short</partinfo>
  
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This part is used to form SpyTag-SpyCatcher hydrogels, and this part immobilizes Metallothioneins in the hydrogel. There are Glycine-Serine linkers between the Metallothioneins and SpyTag.  
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'''This part is not compatible with BioBrick RFC10 assembly but is compatible with the iGEM Type IIS Part standard [[Help:Standards/Assembly/Type_IIS|which is also accepted by iGEM.]]'''
  
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<!-- Add more about the biology of this part here
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==Usage and Biology==
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===Usage and Biology===
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The SpyTag-SpyCatcher protein pair is a modular assembly system. SpyTag and SpyCatcher spontaneously form a covalent interaction with each other. There are Glycine-Serine linkers between the Metallothioneins and SpyTag. This part is used to form SpyTag-SpyCatcher hydrogels, and this part immobilizes Metallothioneins in the hydrogel.
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SpyTag-MT-SpyTag is designed to be a cross linker between triple SpyCatcher subunits for hydrogel formation, with metallothionein (MT) acting as the spacer protein between two SpyTags. This also allows covalent immobilisation of MTs to the SpyTag-SpyCatcher hydrogel.
  
 
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<span class='h3bb'>Sequence and Features</span>
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==<span class='h3bb'>Sequence and Features</span>==
 
<partinfo>BBa_K4390034 SequenceAndFeatures</partinfo>
 
<partinfo>BBa_K4390034 SequenceAndFeatures</partinfo>
  

Revision as of 15:54, 12 October 2022


SpyTag flanked M. edulis Metallothionein

This part is not compatible with BioBrick RFC10 assembly but is compatible with the iGEM Type IIS Part standard which is also accepted by iGEM.

Usage and Biology

The SpyTag-SpyCatcher protein pair is a modular assembly system. SpyTag and SpyCatcher spontaneously form a covalent interaction with each other. There are Glycine-Serine linkers between the Metallothioneins and SpyTag. This part is used to form SpyTag-SpyCatcher hydrogels, and this part immobilizes Metallothioneins in the hydrogel.

SpyTag-MT-SpyTag is designed to be a cross linker between triple SpyCatcher subunits for hydrogel formation, with metallothionein (MT) acting as the spacer protein between two SpyTags. This also allows covalent immobilisation of MTs to the SpyTag-SpyCatcher hydrogel.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 111
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 111
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 111
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 111
    Illegal NgoMIV site found at 60
  • 1000
    COMPATIBLE WITH RFC[1000]