Difference between revisions of "Part:BBa K4260111"

 
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===Usage and Biology===
 
===Usage and Biology===
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[[File:RecA_intein_biosensor_animated.gif|500px|thumb|right|<i><b>Fig.x:</b>RecA intein behavior when an EDC is present.</i>]]
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POR LLENAR
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Revision as of 17:21, 27 September 2022


RecA mini intein mediated ESR1 protein with OmpA signal peptide, linker and AmilCP.

This part encodes an intein mediated biosensor, consisting of a divided AmilCP chromoprotein gene, a mutated intein RecA N-terminal and C-terminal domains and a ESR1 Ligand Binding Domain. The RecA intein comes from Mycobacterium Tuberculosis (Mtu) which has been mutated with the purpose of making it more stable.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]