Difference between revisions of "Part:BBa K4150004"

Revision as of 08:34, 11 August 2022

His-OVA

The chicken ovalbumin sequence obtained from KEGG database (Gallus gallus) was codon optimzed for expression in E. coli and synthesized by Integrated DNA Technologies, Inc. with a connection of a His tag and a GS linker at N-terminus. The ovalbumin (OVA) is a glycoprotein, which is a major component of chicken egg whites, and harbors immunogenic properties in vaccination experiments^[1]. The recombinant OVA protein is readily purified in E. coli BL21 system driven by T7 promoter and triggered by IPTG induction.

Reference

↑ Geary TW, Reeves JJ. Production of a genetically engineered inhibin vaccine. Vaccine. 1996 Sep;14(13):1273-9. doi: 10.1016/s0264-410x(96)00014-x. PMID: 8961517.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
INCOMPATIBLE WITH RFC[1000]
Illegal SapI.rc site found at 373

[1] Geary TW, Reeves JJ. Production of a genetically engineered inhibin vaccine. Vaccine. 1996 Sep;14(13):1273-9. doi: 10.1016/s0264-410x(96)00014-x. PMID: 8961517.

[1]

Revision as of 08:33, 11 August 2022 (view source) Jacky CHIN (Talk \| contribs) ← Older edit		Revision as of 08:34, 11 August 2022 (view source) Jacky CHIN (Talk \| contribs) Newer edit →
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−	The chicken ovalbumin sequence obtained from KEGG database ([https://www.genome.jp/entry/gga:396058 Gallus gallus]) was codon optimzed for expression in E. coli and synthesized by Integrated DNA Technologies, Inc. with a connection of a His tag and a GS linker at N-terminus. The ovalbumin (OVA) is a glycoprotein, which is a major component of chicken egg whites, and harbors immunogenic properties in vaccination experiments<ref>Geary TW, Reeves JJ. Production of a genetically engineered inhibin vaccine. Vaccine. 1996 Sep;14(13):1273-9. doi: 10.1016/s0264-410x(96)00014-x. PMID: 8961517.</ref>. . The recombinant OVA protein is readily purified in E. coli BL21 system driven by T7 promoter and triggered by IPTG induction~~<ref>Geary TW, Reeves JJ. Production of a genetically engineered inhibin vaccine. Vaccine. 1996 Sep;14(13):1273-9. doi: 10.1016/s0264-410x(96)00014-x. PMID: 8961517.</ref>~~.	+	The chicken ovalbumin sequence obtained from KEGG database ([https://www.genome.jp/entry/gga:396058 Gallus gallus]) was codon optimzed for expression in E. coli and synthesized by Integrated DNA Technologies, Inc. with a connection of a His tag and a GS linker at N-terminus. The ovalbumin (OVA) is a glycoprotein, which is a major component of chicken egg whites, and harbors immunogenic properties in vaccination experiments<ref>Geary TW, Reeves JJ. Production of a genetically engineered inhibin vaccine. Vaccine. 1996 Sep;14(13):1273-9. doi: 10.1016/s0264-410x(96)00014-x. PMID: 8961517.</ref>. The recombinant OVA protein is readily purified in E. coli BL21 system driven by T7 promoter and triggered by IPTG induction.