Difference between revisions of "Part:BBa K4284016"

Revision as of 10:14, 26 September 2022

T7-C3 promoter

T7-C2 promoter

contribution

The T7 promoter is a sequence of DNA 18bp long and it is recognized by T7 RNA polymerase 1. It is a strong promoter in the prokaryotic system and is usually used to induce protein expression in E. coli BL21(DE3) by adding IPTG. We specially searched the iGEM Biological Parts library for related components and picked BBa_R0085, the T7 promoter. This is a biological part submitted by Antiquity in 2005 with only basic DNA sequence information and simple text description information. This part had been improved by iGEM18_ZJU-China in 2018, and the group iGEM18_ZJU-China introduced several mutations into it and improved the yield of target genes. Our team carried out some other mutant T7 promoters, adding data from protein expression in E. coli BL21(DE3). In addition, through literature research, we found four a new mutant T7 promoter named T7-C3 promoter. We upload the DNA sequence information and basic introduction information in the registry of standard biological parts to provide more choices of protein expression in the prokaryotic system for future iGEM teams.

Figure 2. Gel electrophoresis results of target gene fragments..

Sequence and Features