Difference between revisions of "Part:BBa K4137002"
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BBa_K4137002, nicknamed mleR for the purposes of our project, is a malate-binding transcriptional activator belonging to l. lactis that has a 6xHis tag attached to permit protein purification. The mleR transcriptional activator portion of the part is responsible for governing malolactic fermentation in l. lactis, and responds to malate to induce malolactic enzyme production. | BBa_K4137002, nicknamed mleR for the purposes of our project, is a malate-binding transcriptional activator belonging to l. lactis that has a 6xHis tag attached to permit protein purification. The mleR transcriptional activator portion of the part is responsible for governing malolactic fermentation in l. lactis, and responds to malate to induce malolactic enzyme production. | ||
+ | [[File:ccda-his.png|800px|thumb|center|Fig.1 ccdA with C-Terminal 6x His-Tag.]] | ||
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Revision as of 04:38, 9 October 2022
Malate-binding Transcriptional Activator + 6xHis
BBa_K4137002, nicknamed mleR for the purposes of our project, is a malate-binding transcriptional activator belonging to l. lactis that has a 6xHis tag attached to permit protein purification. The mleR transcriptional activator portion of the part is responsible for governing malolactic fermentation in l. lactis, and responds to malate to induce malolactic enzyme production.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 18
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]