Difference between revisions of "Part:BBa K4390016"
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__NOTOC__ | __NOTOC__ | ||
<partinfo>BBa_K4390016 short</partinfo> | <partinfo>BBa_K4390016 short</partinfo> | ||
| + | '''This part is not compatible with BioBrick RFC10 assembly but is compatible with the iGEM Type IIS Part standard [[Help:Standards/Assembly/Type_IIS|which is also accepted by iGEM.]]''' | ||
This part is used as a removable His tag for protein purification. This sequence is for use in JUMP assembly and may be incompatible with non-Type IIS assembly techniques, as the fusion site regenerates some nucleotides. | This part is used as a removable His tag for protein purification. This sequence is for use in JUMP assembly and may be incompatible with non-Type IIS assembly techniques, as the fusion site regenerates some nucleotides. | ||
Revision as of 13:11, 12 October 2022
6xHis :: TEV protease cleavage site
This part is not compatible with BioBrick RFC10 assembly but is compatible with the iGEM Type IIS Part standard which is also accepted by iGEM.
This part is used as a removable His tag for protein purification. This sequence is for use in JUMP assembly and may be incompatible with non-Type IIS assembly techniques, as the fusion site regenerates some nucleotides.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]