Difference between revisions of "Part:BBa K4390006"

 
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===Usage and Biology===
 
===Usage and Biology===
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DNA assembly is the cornerstone of synthetic biology, and fast and reliable assembly is a necessity for this. Modular cloning with Type IIS restriction enzymes allows us to quickly assemble many complex multipart constructs from libraries of basic parts. The JUMP vector platform is compatible with the PhytoBrick standard, and vectors are compatible with BioBrick architecture as well as Standard European Vector Architecture (SEVA), and uses single stranded DNA overhangs (fusion sites) generated by BsmBI and BsaI digestion for ordered assembly. There are six different JUMP part types, corresponding to different elements of a transcriptional unit, the Promoter, Ribosome Binding Site (RBS), N-terminus, Open Reading Frame (ORF), C-terminus, and terminator. Figure 1 shows the fusion sites between these part types, and that basic parts can also take up more than one part by adopting a 5’ fusion site of one part and the 3’ fusion site of another.
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[[File:JUMP fusionsites.png|300px|Figure 1: JUMP fusion sites. Taken from [CITATION]. The second and third lines demonstrate how a basic part can adopt fusion sites for different basic parts and be used in assembly as such]]
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===Characterization===
  
 
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Revision as of 22:59, 29 September 2022


JUMP N-filler

This part functions to fill in the space where an N part is meant to go in a JUMP assembly. Effectively, it has N part fusion sites flanking the sequence either side, with as few nucleotides in between as possible to prevent a frameshift mutation.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]