Difference between revisions of "Part:BBa K4161002"
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JMK-H2 is a VHH antibody wich targets the IpaD antigen that makes up the needle tip structure of Type III secretion system of Shigella. The DNA sequence is reversed from the JMK-H2 protein sequence and codon optimized for expression in E. coli. | JMK-H2 is a VHH antibody wich targets the IpaD antigen that makes up the needle tip structure of Type III secretion system of Shigella. The DNA sequence is reversed from the JMK-H2 protein sequence and codon optimized for expression in E. coli. | ||
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+ | Source | ||
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+ | Barta, Michael L., Jonathan P. Shearer, Olivia Arizmendi, Jacqueline M. Tremblay, Nurjahan Mehzabeen, Qi Zheng, Kevin P. Battaile, et al. “Single-Domain Antibodies Pinpoint Potential Targets within Shigella Invasion Plasmid Antigen D of the Needle Tip Complex for Inhibition of Type III Secretion.” Journal of Biological Chemistry 292, no. 40 (October 2017): 16677–87. https://doi.org/10.1074/jbc.M117.802231. | ||
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+ | The sequence can be accessed through RCSB PDB with PDB ID 5VXK. (https://www.rcsb.org/structure/5VXK) | ||
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Revision as of 08:54, 20 July 2022
JMK-H2
JMK-H2 is a VHH antibody wich targets the IpaD antigen that makes up the needle tip structure of Type III secretion system of Shigella. The DNA sequence is reversed from the JMK-H2 protein sequence and codon optimized for expression in E. coli.
Source
Barta, Michael L., Jonathan P. Shearer, Olivia Arizmendi, Jacqueline M. Tremblay, Nurjahan Mehzabeen, Qi Zheng, Kevin P. Battaile, et al. “Single-Domain Antibodies Pinpoint Potential Targets within Shigella Invasion Plasmid Antigen D of the Needle Tip Complex for Inhibition of Type III Secretion.” Journal of Biological Chemistry 292, no. 40 (October 2017): 16677–87. https://doi.org/10.1074/jbc.M117.802231.
The sequence can be accessed through RCSB PDB with PDB ID 5VXK. (https://www.rcsb.org/structure/5VXK)
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]