Difference between revisions of "Part:BBa K4475004:Design"
 
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__NOTOC__
 
__NOTOC__
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<partinfo>BBa_K4475002 short</partinfo>
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<partinfo>BBa_K4475004 short</partinfo>
  
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<partinfo>BBa_K4475002 SequenceAndFeatures</partinfo>
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<partinfo>BBa_K4475004 SequenceAndFeatures</partinfo>
  
  
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===Source===
 
===Source===
  
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Coding sequence from cercospora beticola.
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Genome of cercospora beticola.
  
 
===References===
 
===References===

Latest revision as of 05:40, 19 July 2022


CTB1 Constitutive Weak Protein Generator Yeast


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal XbaI site found at 93
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 4133
    Illegal BamHI site found at 4042
    Illegal BamHI site found at 6215
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal XbaI site found at 93
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal XbaI site found at 93
    Illegal NgoMIV site found at 6722
    Illegal AgeI site found at 4796
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The native coding sequence was trimmed for exon sequences only so that a functional protein would be directly synthesized with minimal gene regulation considerations. The sequence was also codon optimized for yeast and tagged with an N-terminus HA tag.


Source

Genome of cercospora beticola.

References