Difference between revisions of "Part:BBa K4475000:Design"
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===Design Notes===
 
===Design Notes===
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The native sequence was trimmed for exon sequences only so that a functional protein would be directly synthesized with minimal gene regulation considerations. The sequence was also codon optimized for yeast and tagged with an N-terminus HA tag.
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The native coding sequence was trimmed for exon sequences only so that a functional protein would be directly synthesized with minimal gene regulation considerations. The sequence was also codon optimized for yeast and tagged with an N-terminus HA tag.
  
  

Revision as of 05:39, 19 July 2022


CTB1 Gene from Cercospora beticola


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 3532
    Illegal BamHI site found at 3441
    Illegal BamHI site found at 5614
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 6121
    Illegal AgeI site found at 4195
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The native coding sequence was trimmed for exon sequences only so that a functional protein would be directly synthesized with minimal gene regulation considerations. The sequence was also codon optimized for yeast and tagged with an N-terminus HA tag.


Source

Genome of cercospora beticola

References