Difference between revisions of "Part:BBa K4347006"

Revision as of 16:21, 18 July 2022

Sac7e

write small introduction about Sac7e here... eg, what is it? what organism does it come from?

Write about usage and biology... examples used in previous work and difference between the other ssb protiens such as ss07d (part BBa K3790001)

Sac7e was isolated from Sulfolobus acidocaldarius, however Sso7d is a highly similar homolog from Sulfolobus solfataricus.

These proteins are hyper-thermostable and resistant to pH extremes. Sac7e is thermally stable to 85.5°C.

Compared to other similar proteins, Sac7e showed the highest affinity for dsDNA (KD = 11 μM), with binding sites ~ 6-8 bases per protein.

DNA binding protien "Sac7e" modelled in Pymol.

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
INCOMPATIBLE WITH RFC[21]
Illegal BamHI site found at 5
Illegal XhoI site found at 209
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

@@ Line 7: / Line 7: @@
 Write about usage and biology... examples used in previous work and difference between the other ssb protiens such as ss07d (part BBa K3790001)
+Sac7e was isolated from Sulfolobus acidocaldarius, however Sso7d is a highly similar homolog from Sulfolobus solfataricus.
+These proteins are hyper-thermostable and resistant to pH extremes. Sac7e is thermally stable to 85.5°C.
+Compared to other similar proteins, Sac7e showed the highest affinity for dsDNA (KD = 11 μM), with binding sites ~ 6-8 bases per protein.
 [[File:BBa K4347006 sac7e.PNG|300px|center|thumb|DNA binding protien "Sac7e" modelled in Pymol.]]