Difference between revisions of "Part:BBa K091112:Experience"

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===User Reviews===
 
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The MWSU/Davdison iGEM 2009 team sequenced this promoter after there was little to no expression using this it to express GFP and after obtaining what seemed like faulty results during gel verification of its size. The team found that there was a 33 bp insertion within this promoter before the suffix. We believe that the
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E.coli cells may have mutated the promoter to silence its activity in an effort to conserve energy and selection against an attribute that did not necessarily improve its fitness.
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|width='60%' valign='top'|The MWSU/Davdison iGEM 2009 team sequenced this promoter after there was little to no expression using this it to express GFP and after obtaining what seemed like faulty results during gel verification of its size. The team found that there was a 33 bp insertion within this promoter before the suffix. We believe that the
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E.coli cells may have mutated the promoter to silence its activity in an effort to conserve energy and selection against an attribute that did not necessarily improve its fitness.
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Revision as of 21:44, 20 July 2009

This part worked as expected in HB101 cells.
Trial1.png
Trial2.png


Applications of BBa_K091112

User Reviews

The MWSU/Davdison iGEM 2009 team sequenced this promoter after there was little to no expression using this it to express GFP and after obtaining what seemed like faulty results during gel verification of its size. The team found that there was a 33 bp insertion within this promoter before the suffix. We believe that the E.coli cells may have mutated the promoter to silence its activity in an effort to conserve energy and selection against an attribute that did not necessarily improve its fitness.

UNIQfe8973fd33f417d6-partinfo-00000000-QINU UNIQfe8973fd33f417d6-partinfo-00000001-QINU