Difference between revisions of "Part:BBa K4421027"

Revision as of 04:42, 16 July 2022

CAR_library

As we have mentioned in BBa_K4421003, we designed a scFv library with 10 individual colonies. This composite part is formed of 10 kinds of unique CAR constructs based on BBa_K4421003.

Structure of CAR_library

Our design is based on second-generation CAR, consisting of 1)endodomain: CD28 and CD3ζ 2)transmembrane domain: CD8TM 3)ectodomain: CD8 hinge and the scFv library. The unique point of our design is the scFv library, theoretically composed of all kinds of scFvs which can be genetated by human bodies. In our cellular experiment, we selected 10 scFvs to form a small library, which is detailed in BBa_K4421003.

Special Note

Since our part BBa_K4421003 is a general concept without a specific sequence, we use BBa_K4421011 cetuximab scfv to serve as a placeholder. In other words, the place where BBa_K4421011 at can be replaced by any other scFv to form diverse CAR constructs.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
INCOMPATIBLE WITH RFC[21]
Illegal BglII site found at 2042
23
COMPATIBLE WITH RFC[23]
25
INCOMPATIBLE WITH RFC[25]
Illegal NgoMIV site found at 298
Illegal NgoMIV site found at 1832
Illegal NgoMIV site found at 1898
Illegal NgoMIV site found at 2065
1000
INCOMPATIBLE WITH RFC[1000]
Illegal BsaI.rc site found at 1439
Illegal SapI.rc site found at 1996

@@ Line 5: / Line 5: @@
 As we have mentioned in <partinfo>BBa_K4421003</partinfo>, we designed a scFv library with 10 individual colonies. This composite part is formed of 10 kinds of unique CAR constructs based on <partinfo>BBa_K4421003</partinfo>.
-===Structure of CAR-library===
+===Structure of CAR_library===
 Our design is based on second-generation CAR, consisting of 1)endodomain: CD28 and CD3ζ 2)transmembrane domain: CD8TM 3)ectodomain: CD8 hinge and the scFv library. The unique point of our design is the scFv library, theoretically composed of all
 kinds of scFvs which can be genetated by human bodies. In our cellular experiment, we selected 10 scFvs to form a small library, which is detailed in <partinfo>BBa_K4421003</partinfo>.
 ===Special Note===
 Since our part <partinfo>BBa_K4421003</partinfo> is a general concept without a specific sequence, we use <partinfo>BBa_K4421011</partinfo> cetuximab scfv to serve as a placeholder. In other words, the place where <partinfo>BBa_K4421011</partinfo> at can be replaced by any other scFv to form diverse CAR constructs.