Difference between revisions of "Part:BBa K4421027"
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<partinfo>BBa_K4421027 short</partinfo>
 
<partinfo>BBa_K4421027 short</partinfo>
  
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As we have mentioned in BBa_K4421003, we designed a scfv library with 10 individual colonies.This composite part is formed of 10 kinds of unique CAR constructs based on BBa_K4421003.
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As we have mentioned in <partinfo>BBa_K4421003</partinfo>, we designed a scFv library with 10 individual colonies. This composite part is formed of 10 kinds of unique CAR constructs based on <partinfo>BBa_K4421003</partinfo>.
 
===Special Note===
 
===Special Note===
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Since our part BBa_K4421003 is a general concept without a specific sequence, we use BBa_K4421011 to serve as a placeholder.In other word, the place where BBa_K4421011 at can be replaced by any other scFv to form diverse CAR constructs.
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Since our part <partinfo>BBa_K4421003</partinfo> is a general concept without a specific sequence, we use <partinfo>BBa_K4421011</partinfo> to serve as a placeholder. In other words, the place where <partinfo>BBa_K4421011</partinfo> at can be replaced by any other scFv to form diverse CAR constructs.
 
<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here
 
===Usage and Biology===
 
===Usage and Biology===

Revision as of 04:04, 16 July 2022


CAR_library

As we have mentioned in BBa_K4421003, we designed a scFv library with 10 individual colonies. This composite part is formed of 10 kinds of unique CAR constructs based on BBa_K4421003.

Special Note

Since our part BBa_K4421003 is a general concept without a specific sequence, we use BBa_K4421011 to serve as a placeholder. In other words, the place where BBa_K4421011 at can be replaced by any other scFv to form diverse CAR constructs.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 2042
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 298
    Illegal NgoMIV site found at 1832
    Illegal NgoMIV site found at 1898
    Illegal NgoMIV site found at 2065
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1439
    Illegal SapI.rc site found at 1996