Difference between revisions of "Part:BBa K4347007:Design"

 
Line 7: Line 7:
  
 
===Design Notes===
 
===Design Notes===
−
N/A
+
How we came up with these residues to mutate.
−
 
+
Making point mutations in yasara - talking about the differences in gibbs free energy.
  
 +
===Design Considerations===
 +
Considering which residues were in active site or not. Fingers are important for strand displacement and activity in taq(victor to fetch paper) and when mutated only 25% worked compared to thumb and palm domains.
  
 
===Source===
 
===Source===
  
−
Sulfolobus acidocaldarius
+
PDB: 6mu5 (insert link)
  
 
===References===
 
===References===

Revision as of 15:01, 8 July 2022


Bst with point mutations for enhanced thermal stability codon optimized for E.coli


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 766
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

How we came up with these residues to mutate. Making point mutations in yasara - talking about the differences in gibbs free energy.

Design Considerations

Considering which residues were in active site or not. Fingers are important for strand displacement and activity in taq(victor to fetch paper) and when mutated only 25% worked compared to thumb and palm domains.

Source

PDB: 6mu5 (insert link)

References