Difference between revisions of "Part:BBa K176030:Design"

(→‎Design Notes)
 
Line 14: Line 14:
 
Design:
 
Design:
  
−
Input1 PoPS generate TetR-LVA
+
Input1 PoPS generates TetR-LVA
  
−
pCon 0.70(J23101) generate LuxR
+
pCon 0.70(J23101) generates LuxR
  
−
pLux/Tet(K176000) generate GFP, activated by LuxR and Input2 AHL, repressed by TetR-LVA and no aTc
+
pLux/Tet(K176000) generates GFP, activated by LuxR and Input2 AHL, repressed by TetR-LVA and no aTc
  
 
===Source===
 
===Source===

Latest revision as of 06:27, 30 July 2009

Repressible AHL->GFP Receiver: PoPS->tetR-LVA+pCon 0.70->luxR+pLux/Tet->GFP


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 855
    Illegal NheI site found at 878
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1833
    Illegal BsaI.rc site found at 2577


Design Notes

Input1: PoPS

Input2: AHL

Output: GFP

Design:

Input1 PoPS generates TetR-LVA

pCon 0.70(J23101) generates LuxR

pLux/Tet(K176000) generates GFP, activated by LuxR and Input2 AHL, repressed by TetR-LVA and no aTc

Source

K176006 K176026

References