Difference between revisions of "Part:BBa K4421021"
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It consists of six NFAT-RE sites. NFAT-RE can bind with NFAT factors, thus activating expression of the downstream genes, which is original from the IL-2 promotor. | It consists of six NFAT-RE sites. NFAT-RE can bind with NFAT factors, thus activating expression of the downstream genes, which is original from the IL-2 promotor. | ||
The regulation of IL-2 gene was revealed comprehensively during last century. | The regulation of IL-2 gene was revealed comprehensively during last century. | ||
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<span class='h3bb'>Sequence and Features</span> | <span class='h3bb'>Sequence and Features</span> | ||
Revision as of 06:02, 7 June 2022
NFAT-RE promotor
This part is a CAR inducible promoter containing six NFAT-RE sites in a minimal IL-2 promoter to generate CAR response elements, which was placed upstream of the transcription factor Gal4-KRAB.In our engineered NK-92 cells, NFAT was coupled with KRAB activation after CAR stimulation.
Usage and Biology
This part can serve as a downstream promotor of CAR recognition in synthetic immunology, especially in constructing CAR-classis cells with unique circus.Together with synnotch,we can really do a lot.
supplementary information
It consists of six NFAT-RE sites. NFAT-RE can bind with NFAT factors, thus activating expression of the downstream genes, which is original from the IL-2 promotor. The regulation of IL-2 gene was revealed comprehensively during last century.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]