Difference between revisions of "Part:BBa K4421003"

Revision as of 12:30, 26 May 2022

scfv library with 10 individual colonies

This part is a phage display scfv library which consists of ten kinds of individual colonies.The sequence encoding the scFv antibodies generated from cetuximab,trastuzumab, CH65, 9.8B, 2F5, F10, 7D11, 8D6, omalizumab, TE33, R10 and HC33.8 was chemically synthesized. The sequence information can be found in the Protein Data Bank. And we next aim to construct a CAR-NK-92 cell library of 10 kinds of unique CAR constructs with this part.

Usage and Biology

Antibody libraries can be constructed based on different sources, such as immunized animals or naturally immunized or infected humans, or naive immune systems, which can be derived from nonimmune natural or computational and synthetic sources.In vitro experiments, the MCF-7 cells and the derivative cells were characterized, which have been used in our previous study.A small library of 10 CAR constructs comprising antigen-specific scFv(BBa K4421003) were constructed, termed as the NK-92CTX-TTZ library.And the sequence encoding the scFv antibodies was generated from cetuximab(CTX), trastuzumab(TTZ), CH65, 9.8B, 2F5, F10, 7D11, 8D6, omalizumab, TE33, R10 and HC33.8.

Background and detail description

Used for a CAR

In. our project,

Sequence and Features