Difference between revisions of "Part:BBa K116401"

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====Experience (Lambert_GA 2021)====
 
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Utilizing the modified phosphate range and characterization protocol (See: [https://2021.igem.org/Team:Lambert_GA/Pho#:~:text=Engineering%20Success%20page.-,Characterization%20Protocol,-The%20failed%20characterization Modified Protocol]), we characterized the BBa_K2447000 phosphate sensor. The characterization curve (Figure 1) showed a linear, negative trend throughout phosphate concentrations ranging from 0-100μM. This data closely paralleled the predictive ODE model created by our 2020 team and the previous characterization data by NUS Singapore iGEM 2017.
 
  
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[[File:Fjasknfkncjak.png|thumb|center|800px|Figure 1. Characterization curve for BBa_K2447000 for phosphate concentrations between 0μM and 100μM. ]]
 
 
<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here
  

Latest revision as of 19:50, 17 December 2021


external phosphate sensing promoter

Promoter of phoB in E.coli, an external phosphate regulated promoter that can be activated when encountering phosphate limitation.



Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]