Difference between revisions of "Part:BBa K4060105:Experience"
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====Assembly of PreB2 (pSB1C3)====
 
====Assembly of PreB2 (pSB1C3)====
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The gene fragment Pre B2 is composed of Pre A2 and the Thermometer RBS <partinfo>BBa_K115002</partinfo>. We digested the vector fragment <partinfo>BBa_K115002</partinfo> with EcoRI and XbaI and the insert fragment Pre A2 with EcoRI and SpeI. We ligate these fragments together to form Pre B2.
 
  
 
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Fig.6 Agarose electrophoresis in order to verify Pre B2. Lane 1, 100bp ladder.Lane 1’, partial of Life 1 kb Plus ladder. Lane 2,3, Pre B2 digested with NotI.
 
Fig.6 Agarose electrophoresis in order to verify Pre B2. Lane 1, 100bp ladder.Lane 1’, partial of Life 1 kb Plus ladder. Lane 2,3, Pre B2 digested with NotI.
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===Applications of BBa_K4060105===
 
===Applications of BBa_K4060105===

Latest revision as of 14:27, 7 December 2021


Assembly of PreB2 (pSB1C3)

Fig.6 Agarose electrophoresis in order to verify Pre B2. Lane 1, 100bp ladder.Lane 1’, partial of Life 1 kb Plus ladder. Lane 2,3, Pre B2 digested with NotI.

Applications of BBa_K4060105

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UNIQ5858e619016a5e50-partinfo-00000001-QINU UNIQ5858e619016a5e50-partinfo-00000002-QINU