Difference between revisions of "Part:BBa K4060104:Experience"
Clairechen (Talk | contribs) (→Assembly of Pre B1 (pSB1C3)) |
Clairechen (Talk | contribs) (→Assembly of Pre B1 (pSB1C3)) |
||
Line 8: | Line 8: | ||
</body></html> | </body></html> | ||
− | Fig.1 Agarose electrophoresis in order to verify Pre B1. | + | Fig.1 Agarose electrophoresis in order to verify Pre B1. Lane 1, 100bp ladder.Lane 1’, partial of Life 1 kb Plus ladder. Lane 2,3, Pre B1 digested with NheI and PstI. |
===Applications of BBa_K4060104=== | ===Applications of BBa_K4060104=== |
Revision as of 14:19, 7 December 2021
Assembly of Pre B1 (pSB1C3)
The gene fragment Pre B1 is composed of Pre A1 and the TetR gene BBa_C0040. We digested the vector fragment Pre A1 with SpeI and PstI; the insert fragment BBa_C0040 with XbaI and PstI. We ligated these fragments together to form Pre B1.
![](https://2021.igem.org/wiki/images/8/88/T--NYCU-Taipei--image9.png)
Fig.1 Agarose electrophoresis in order to verify Pre B1. Lane 1, 100bp ladder.Lane 1’, partial of Life 1 kb Plus ladder. Lane 2,3, Pre B1 digested with NheI and PstI.
Applications of BBa_K4060104
User Reviews
UNIQ1a5c0a759bb57a5a-partinfo-00000003-QINU UNIQ1a5c0a759bb57a5a-partinfo-00000004-QINU