Difference between revisions of "Part:BBa K4060101"
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<partinfo>BBa_K4060101 short</partinfo> | <partinfo>BBa_K4060101 short</partinfo> | ||
− | + | The gene fragment Pre A2 is composed of pTetR(BBa_R0040) and a double terminator(BBa_B0015). We digested the vector fragment BBa_R0040 with XbaI and EcoRI and the insert fragment BBa_B0015 with EcoRI and SpeI. We ligated these fragments together to form Pre A2. | |
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Revision as of 14:23, 7 December 2021
[Pre A2] double terminator + pTetR
The gene fragment Pre A2 is composed of pTetR(BBa_R0040) and a double terminator(BBa_B0015). We digested the vector fragment BBa_R0040 with XbaI and EcoRI and the insert fragment BBa_B0015 with EcoRI and SpeI. We ligated these fragments together to form Pre A2.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]