Difference between revisions of "Part:BBa K3844004:Sequence, Features, and Subparts"
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Latest revision as of 03:50, 22 October 2021
1. Function
This is another transcription factor that also binds to the Styrene VOC. It mainly regulates and represses the transcription of certain genes.
2. Design Consideration
Our project requires for the transcription factor to be dependent on the presence of VOCs. When researching the design of trimethylbenzene, the VOC that this transcription factor binds to, we needed to make sure that, hypothetically, everything would work based on our model. After checking through extensive research that our design would fit with this transcription factor, we chose it.
3. Characterization
Sequence:atgaccaccctggatagcaacaacaacaccggcggcgtgattacctatattggcagcagc ggcagcagcccgagccgcaccagcccggaaagcctgtatagcgataacagcaacggcagc tttcagagcctgacccagggctgcccgacctattttccgccgagcccgaccggcagcctg acccaggatccggcgcgcagctttggcagcattccgccgagcctgagcgatgatggcagc ccgagcagcagcagcagcagcagcagcagcagcagcagcttttataacggcagcccgccg ggcagcctgcaggtggcgatggaagatagcagccgcgtgagcccgagcaaaagcaccagc aacattaccaaactgaacggcatggtgctgctgtgcaaagtgtgcggcgatgtggcgagc ggctttcattatggcgtgcatgcgtgcgaaggctgcaaaggcttttttcgccgcagcatt cagcagaacattcagtataaacgctgcctgaaaaacgaaaactgcagcattgtgcgcatt aaccgcaaccgctgccagcagtgccgctttaaaaaatgcctgagcgtgggcatgagccgc gatgcggtgcgctttggccgcattccgaaacgcgaaaaacagcgcatgctggcggaaatg cagagcgcgatgaacctggcgaacaaccagctgagcagccagtgcccgctggaaaccagc ccgacccagcatccgaccccgggcccgatgggcccgagcccgccgccggcgccggtgccg agcccgctggtgggctttagccagtttccgcagcagctgaccccgccgcgcagcccgagc ccggaaccgaccgtggaagatgtgattagccaggtggcgcgcgcgcatcgcgaaattttt acctatgcgcatgataaactgggcagcagcccgggcaactttaacgcgaaccatgcgagc ggcagcccgccggcgaccaccccgcatcgctgggaaaaccagggctgcccgccggcgccg aacgataacaacaccctggcggcgcagcgccataacgaagcgctgaacggcctgcgccag gcgccgagcagctatccgccgacctggccgccgggcccggcgcatcatagctgccatcag agcaacagcaacggccatcgcctgtgcccgacccatgtgtatgcggcgccggaaggcaaa gcgccggcgaacagcccgcgccagggcaacagcaaaaacgtgctgctggcgtgcccgatg aacatgtatccgcatggccgcagcggccgcaccgtgcaggaaatttgggaagattttagc atgagctttaccccggcggtgcgcgaagtggtggaatttgcgaaacatattccgggcttt cgcgatctgagccagcatgatcaggtgaccctgctgaaagcgggcacctttgaagtgctg atggtgcgctttgcgagcctgtttaacgtgaaagatcagaccgtgatgtttctgagccgc accacctatagcctgcaggaactgggcgcgatgggcatgggcgatctgctgagcgcgatg tttgattttagcgaaaaactgaacagcctggcgctgaccgaagaagaactgggcctgttt accgcggtggtgctggtgagcgcggatcgcagcggcatggaaaacagcgcgagcgtggaa cagctgcaggaaaccctgctgcgcgcgctgcgcgcgctggtgctgaaaaaccgcccgctg gaaaccagccgctttaccaaactgctgctgaaactgccggatctgcgcaccctgaacaac Atgcatagcgaaaaactgctgagctttcgcgtggatgcgcag
4. References
1. https://www.genecards.org/cgi-bin/carddisp.pl?gene=NR1D1
2. https://www.ncbi.nlm.nih.gov/gene/9572
3. https://www.uniprot.org/uniprot/P20393
Sequence and Features
- 10INCOMPATIBLE WITH RFC[10]Illegal PstI site found at 307
Illegal PstI site found at 522
Illegal PstI site found at 1513
Illegal PstI site found at 1684 - 12INCOMPATIBLE WITH RFC[12]Illegal PstI site found at 307
Illegal PstI site found at 522
Illegal PstI site found at 1513
Illegal PstI site found at 1684
Illegal NotI site found at 1283 - 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 186
- 23INCOMPATIBLE WITH RFC[23]Illegal PstI site found at 307
Illegal PstI site found at 522
Illegal PstI site found at 1513
Illegal PstI site found at 1684 - 25INCOMPATIBLE WITH RFC[25]Illegal PstI site found at 307
Illegal PstI site found at 522
Illegal PstI site found at 1513
Illegal PstI site found at 1684
Illegal NgoMIV site found at 765
Illegal NgoMIV site found at 969
Illegal NgoMIV site found at 1011
Illegal NgoMIV site found at 1203 - 1000COMPATIBLE WITH RFC[1000]