Difference between revisions of "Part:BBa K4060106"
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===Usage and Biology=== | ===Usage and Biology=== | ||
− | + | The RFP expression of Construct 3 | |
− | + | We used RFP(E1010) to monitor the expression of TetR, so that we can figure out how L-arabinose will influence the expression of TetR in our Design 1. | |
+ | <div class="img"> | ||
+ | <img src="https://2021.igem.org/File:T--NYCU-Taipei--kilksw_result_1.png" alt=""> | ||
+ | </div> | ||
+ | |||
+ | <div class="img"> | ||
+ | <img src="https://2021.igem.org/File:T--NYCU-Taipei--kilksw_result_2.png" alt=""> | ||
+ | </div> | ||
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<span class='h3bb'>Sequence and Features</span> | <span class='h3bb'>Sequence and Features</span> |
Revision as of 03:44, 22 October 2021
Pre B3 gene
a kill switch strategy.
Usage and Biology
The RFP expression of Construct 3 We used RFP(E1010) to monitor the expression of TetR, so that we can figure out how L-arabinose will influence the expression of TetR in our Design 1.
<img src="https://2021.igem.org/File:T--NYCU-Taipei--kilksw_result_1.png" alt="">
<img src="https://2021.igem.org/File:T--NYCU-Taipei--kilksw_result_2.png" alt="">
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 125
- 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 65
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 711
Illegal AgeI site found at 823 - 1000COMPATIBLE WITH RFC[1000]