Difference between revisions of "Part:BBa K3844002:Sequence, Features, and Subparts"

Revision as of 03:15, 22 October 2021

1. Function

Also known as mimR, this is a transcriptional regulator. It specializes in multiple aspects of synthetic biology, including: ATP-binding, sequence-specific DNA binding, and transcription factor binding.

2. Design Consideration

Our project requires for the transcription factor to be dependent on the presence of VOCs. When researching the design of trimethylbenzene, the VOC that this transcription factor binds to, we needed to make sure that, hypothetically, everything would work based on our model. After checking through extensive research that our design would fit with this transcription factor, we chose it.

3. Characterization

Sequence: atggtgcgccatgcggataccccgaccgatgcgcagcgcgcgcgcgaaca gtttctgagcaccggcgcgctgcagccggatgcggtggcgagcagcgtgctgaacagctg gcagcgcagccgcgaactgcatgtgcatccggatcgcgtggaactgccgtatgtgcgcga tccggataccgataccccgctgatgcatgcggcggcgccggtgctgcgccgcatggcgga agatctgagcgatcagagcgtgagcgtggtgctgaccagcgcggatggcctggtgctgga tcgcgtggcggcggataccgaatttgaacgcgtgctggatgatgtgcgcctggcgcgcgg ctatagctatgcggaagaatttgcgggcaccaacggcattggcaccgcgctggaaaccgg ccgcccggcgtttattcgcggcagcgaacattatgtgggcaccctgggcggcctggcgtg cgcgggcagcccgattcgcgaaccggtgacccgccgcattctgggcgtgattgatctgac ctgccgcgcgcgccaggcggatccgctgctgtttgtgctggcgaaaagcgcgggcagcca gattgaagatcgcctgcgcaccatgaacaacgaaaccgaaaccgcgctgctggatgcgta tctgaaacagagccgccgctatccgggcggcgtgctggcgattggcggcgatgtggtgct gatgaaccgctatctgcgccaggcgctggatgcggcggatcagaccgtgctgctggatca tgcggcggaactgacccgcagcagctttaccagcaccaccgtggcgcatctgccgagcgg cgcgagcgtgaaaattagcgcggcggaacgcattattgtgggcgtgcgcagcgatagcgt ggtgtttcatgtgagcgtgcatgtggcggaaagcattccggtgcgcagcagccagcatat tccgcgcctggcgggccagagcagcagctttcgccgcagcgcgcagcaggtggaacgctg ctatcgcgatcgcgaatgggtggtgattgaaggcgaacgcggcagcggccgcacctatct gggctatagcgtggtgcattttgtgaccccggaacgcagcattccggtgctgcatattga taactttgataccccggaaagctttgtggcggcgtttgaaagcgaaaccgatagcgcgga ttttgcggtgattgtgagcgatgtggatgaactgccggatgaagtgctgaacccgatggc ggcgattatgcagacccgcgcgggccgcggctggattgcggcgaccaccagcaccgaacg cgatagccagctggtggatctgctgatgctgccgttttttacccataccgtgaccgtgcc ggcgctgcgccatcgcattgaagatctgcaggaactggtgccgatgattctgaacgaact gagccgcggcgaagcgcgcatggatggcgaagcgatgcgccagctggcgaaactgccgtg gccgggcaacattgcgcagctgcgccatgtgctgaccgaaaccgtgcgccgccagcgcag cggcgtgattggcgtggataaactgccggcggaatgccgcagcgtgacccgccgcaaact gaccccgctggaagcgatggaacgcgatgcgattgtgcgcagcctgctggaaaacgatgg caacaaagcggatgcggcggcggcgctgggcatgagccgcgcgaccatttatcgcaaaat taaagattttggcattgcg

4. References

1. https://www.uniprot.org/uniprot/G3XGC1

2. https://www.uniprot.org/uniprot/A0QTU7.fasta

3. https://www.bioinformatics.org/sms2/rev_trans.html

Sequence and Features