Difference between revisions of "Part:BBa K3792006"
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Chitin from the cells was converted into chitosan by reacting with 12.5M NaOH at 95°C for 24 hours. The solution was then neutralized with acetic acid. The assay was then performed on the supernatant of the cell culture. | Chitin from the cells was converted into chitosan by reacting with 12.5M NaOH at 95°C for 24 hours. The solution was then neutralized with acetic acid. The assay was then performed on the supernatant of the cell culture. | ||
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<td style="--size: calc(897/900)"><span class="tooltip">iGEM: [https://parts.igem.org/Part:BBa_M36303 BBa_M36303]<br>BIOFAB: apFAB46<br>Strength: 897</span><span class="data">897</span></td> | <td style="--size: calc(897/900)"><span class="tooltip">iGEM: [https://parts.igem.org/Part:BBa_M36303 BBa_M36303]<br>BIOFAB: apFAB46<br>Strength: 897</span><span class="data">897</span></td> |
Revision as of 01:08, 22 October 2021
NodCIJ Generator I
The NodCIJ Generator I has a BIOFAB promoter with a strength recorded at x fluorescence per cell. The strongest promoter in the collection has a strength of y . NodC is a chitin synthase that produces tetrameric chitooligomers. NodI and NodJ are transport proteins that usually secrete.
This part consists of 2555 base-pair length. It includes a promoter, 3 ribosome binding sites, 3 protein-coding sequences, and a terminator. We used promoter K2832184, a weak promoter from the BIOFAB collection. In addition, we also used protein-coding sequences from Chitin synthase from Rhiozobium leguminosarum, Nodl a protein possibly involved in transporting chitin across the cell membrane, and NodJ, a protein also possibly involved in the transportation of chitin
This generator was assembled into the pSB1K3 plasmid, and then transformed into E.Coli NEB 5-Alpha. To test if the cells were secreting chitin, The FSU 2021 team used the Chitosan Assay Kit (XAN-5126) available from Cell Biolabs. The manual for this kit contains the protocols for converting chitin to chitosan, along with a protocol for detecting chitosan.
Chitin from the cells was converted into chitosan by reacting with 12.5M NaOH at 95°C for 24 hours. The solution was then neutralized with acetic acid. The assay was then performed on the supernatant of the cell culture.
iGEM: BBa_M36303 BIOFAB: apFAB46 Strength: 897897 |
iGEM: BBa_K2832101 BIOFAB: apFAB70 Strength: 866.7866.7 |
iGEM: BBa_K2832102 BIOFAB: apFAB71 Strength: 866866 |
iGEM: BBa_K2832103 BIOFAB: apFAB61 Strength: 857857 |
Sequence and Features
- 10INCOMPATIBLE WITH RFC[10]Unknown
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Unknown
- 23INCOMPATIBLE WITH RFC[23]Unknown
- 25INCOMPATIBLE WITH RFC[25]Unknown
- 1000INCOMPATIBLE WITH RFC[1000]Illegal SapI.rc site found at 854